antimouse cd11c antibody Search Results


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Integrin Alpha X, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio anti cd11c  (Miltenyi Biotec)
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Miltenyi Biotec bio anti cd11c
Bio Anti Cd11c, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse cd11c antibody  (Miltenyi Biotec)
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Anti Mouse Cd11c Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percp vio 700  (Miltenyi Biotec)
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biotinylated n418 mab  (Miltenyi Biotec)
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anti cd11c mab  (Miltenyi Biotec)
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Miltenyi Biotec anti cd11c mab
Anti Cd11c Mab, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe conjugated cd11c antibody  (Miltenyi Biotec)
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Miltenyi Biotec pe conjugated cd11c antibody
In vivo detection of the red fluorescent protein in different organs of vaccinated NOD mice. NOD mice were given a single oral dose of Salmonella -containing plasmid (pSBbi-RP/SB100, n = 10) compared to Salmonella only ( n = 10). Four weeks post-vaccination, red fluorescent protein was detected in different organs. (A) Representative FACS plots gated the frequency of live red-labeled <t>CD11c</t> + and CD11c – cells in the spleens, pancreatic lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer’s patches (PP) of mice from each group. (B) Quantification of the percentages of the CD11c + and CD11c – red-labeled cells in all organs. (C) Quantification of the percentages of red-labeled CD11c + MHCII + in total live cells isolated from the spleen, PLN, MLN, and PP. The data shown are as the average with standard deviation (SD) obtained from two independent experiments. Statistical analysis using two-way ANOVA shows significance between groups (**** p < 0.0001).
Pe Conjugated Cd11c Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd11c pe vio770  (Miltenyi Biotec)
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Miltenyi Biotec anti cd11c pe vio770
In vivo detection of the red fluorescent protein in different organs of vaccinated NOD mice. NOD mice were given a single oral dose of Salmonella -containing plasmid (pSBbi-RP/SB100, n = 10) compared to Salmonella only ( n = 10). Four weeks post-vaccination, red fluorescent protein was detected in different organs. (A) Representative FACS plots gated the frequency of live red-labeled <t>CD11c</t> + and CD11c – cells in the spleens, pancreatic lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer’s patches (PP) of mice from each group. (B) Quantification of the percentages of the CD11c + and CD11c – red-labeled cells in all organs. (C) Quantification of the percentages of red-labeled CD11c + MHCII + in total live cells isolated from the spleen, PLN, MLN, and PP. The data shown are as the average with standard deviation (SD) obtained from two independent experiments. Statistical analysis using two-way ANOVA shows significance between groups (**** p < 0.0001).
Anti Cd11c Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd11c apc  (Miltenyi Biotec)
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Miltenyi Biotec cd11c apc
In vivo detection of the red fluorescent protein in different organs of vaccinated NOD mice. NOD mice were given a single oral dose of Salmonella -containing plasmid (pSBbi-RP/SB100, n = 10) compared to Salmonella only ( n = 10). Four weeks post-vaccination, red fluorescent protein was detected in different organs. (A) Representative FACS plots gated the frequency of live red-labeled <t>CD11c</t> + and CD11c – cells in the spleens, pancreatic lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer’s patches (PP) of mice from each group. (B) Quantification of the percentages of the CD11c + and CD11c – red-labeled cells in all organs. (C) Quantification of the percentages of red-labeled CD11c + MHCII + in total live cells isolated from the spleen, PLN, MLN, and PP. The data shown are as the average with standard deviation (SD) obtained from two independent experiments. Statistical analysis using two-way ANOVA shows significance between groups (**** p < 0.0001).
Cd11c Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd11c  (Miltenyi Biotec)
95
Miltenyi Biotec anti cd11c
In vivo detection of the red fluorescent protein in different organs of vaccinated NOD mice. NOD mice were given a single oral dose of Salmonella -containing plasmid (pSBbi-RP/SB100, n = 10) compared to Salmonella only ( n = 10). Four weeks post-vaccination, red fluorescent protein was detected in different organs. (A) Representative FACS plots gated the frequency of live red-labeled <t>CD11c</t> + and CD11c – cells in the spleens, pancreatic lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer’s patches (PP) of mice from each group. (B) Quantification of the percentages of the CD11c + and CD11c – red-labeled cells in all organs. (C) Quantification of the percentages of red-labeled CD11c + MHCII + in total live cells isolated from the spleen, PLN, MLN, and PP. The data shown are as the average with standard deviation (SD) obtained from two independent experiments. Statistical analysis using two-way ANOVA shows significance between groups (**** p < 0.0001).
Anti Cd11c, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd11c vioblue  (Miltenyi Biotec)
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Miltenyi Biotec cd11c vioblue
List of cell surface markers used in this study
Cd11c Vioblue, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd11c  (Miltenyi Biotec)
95
Miltenyi Biotec cd11c
List containing all antibodies utilized for surface staining of mass cytometry samples.
Cd11c, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


In vivo detection of the red fluorescent protein in different organs of vaccinated NOD mice. NOD mice were given a single oral dose of Salmonella -containing plasmid (pSBbi-RP/SB100, n = 10) compared to Salmonella only ( n = 10). Four weeks post-vaccination, red fluorescent protein was detected in different organs. (A) Representative FACS plots gated the frequency of live red-labeled CD11c + and CD11c – cells in the spleens, pancreatic lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer’s patches (PP) of mice from each group. (B) Quantification of the percentages of the CD11c + and CD11c – red-labeled cells in all organs. (C) Quantification of the percentages of red-labeled CD11c + MHCII + in total live cells isolated from the spleen, PLN, MLN, and PP. The data shown are as the average with standard deviation (SD) obtained from two independent experiments. Statistical analysis using two-way ANOVA shows significance between groups (**** p < 0.0001).

Journal: Frontiers in Immunology

Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice

doi: 10.3389/fimmu.2020.00712

Figure Lengend Snippet: In vivo detection of the red fluorescent protein in different organs of vaccinated NOD mice. NOD mice were given a single oral dose of Salmonella -containing plasmid (pSBbi-RP/SB100, n = 10) compared to Salmonella only ( n = 10). Four weeks post-vaccination, red fluorescent protein was detected in different organs. (A) Representative FACS plots gated the frequency of live red-labeled CD11c + and CD11c – cells in the spleens, pancreatic lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer’s patches (PP) of mice from each group. (B) Quantification of the percentages of the CD11c + and CD11c – red-labeled cells in all organs. (C) Quantification of the percentages of red-labeled CD11c + MHCII + in total live cells isolated from the spleen, PLN, MLN, and PP. The data shown are as the average with standard deviation (SD) obtained from two independent experiments. Statistical analysis using two-way ANOVA shows significance between groups (**** p < 0.0001).

Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using PE-conjugated CD11c antibody (Miltenyi, 130-110-701) followed by anti-PE microbeads (Miltenyi Biotec, 130-091-041).

Techniques: In Vivo, Plasmid Preparation, Labeling, Isolation, Standard Deviation

Vaccine-delivered TGFβ alters levels of DCs in secondary lymphoid tissues. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100, n = 10) or Salmonella (pSBbi-RP/SB100, n = 10). Four weeks post-vaccination, red fluorescent protein was detected in DCs of different organs. (A) Representative FACS plots gated the frequency of MHCII + CD11c + Td-Tomato + cells in the spleens, PLN, MLN, and PP of mice from each group. (B) Quantification of the percentages of MHCII + CD11c + Td-Tomato + cells in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (*** p < 0.001, **** p < 0 0001).

Journal: Frontiers in Immunology

Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice

doi: 10.3389/fimmu.2020.00712

Figure Lengend Snippet: Vaccine-delivered TGFβ alters levels of DCs in secondary lymphoid tissues. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100, n = 10) or Salmonella (pSBbi-RP/SB100, n = 10). Four weeks post-vaccination, red fluorescent protein was detected in DCs of different organs. (A) Representative FACS plots gated the frequency of MHCII + CD11c + Td-Tomato + cells in the spleens, PLN, MLN, and PP of mice from each group. (B) Quantification of the percentages of MHCII + CD11c + Td-Tomato + cells in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (*** p < 0.001, **** p < 0 0001).

Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using PE-conjugated CD11c antibody (Miltenyi, 130-110-701) followed by anti-PE microbeads (Miltenyi Biotec, 130-091-041).

Techniques:

Vaccine-delivered TGFβ alters levels of gut DCs. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100, n = 10) or Salmonella (pSBbi-RP/SB100, n = 10). Four weeks post-vaccination, red fluorescent protein was detected in DCs of different organs. (A) Representative FACS plots gated the frequency of the live red-labeled MHCII + CD11c + CD103 + cells in the spleens, PLN, MLN, and PP of mice from each group. (B) Quantification of the numbers of MHCII + CD11c + CD103 + in red-labeled cells in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (**** p < 0.0001).

Journal: Frontiers in Immunology

Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice

doi: 10.3389/fimmu.2020.00712

Figure Lengend Snippet: Vaccine-delivered TGFβ alters levels of gut DCs. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100, n = 10) or Salmonella (pSBbi-RP/SB100, n = 10). Four weeks post-vaccination, red fluorescent protein was detected in DCs of different organs. (A) Representative FACS plots gated the frequency of the live red-labeled MHCII + CD11c + CD103 + cells in the spleens, PLN, MLN, and PP of mice from each group. (B) Quantification of the numbers of MHCII + CD11c + CD103 + in red-labeled cells in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (**** p < 0.0001).

Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using PE-conjugated CD11c antibody (Miltenyi, 130-110-701) followed by anti-PE microbeads (Miltenyi Biotec, 130-091-041).

Techniques: Labeling

Endogenous TGFβ alters PDL-1 and CTLA4 expression in secondary lymphoid tissues. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100) or Salmonella (pSBbi-RP/SB100). Four weeks post-vaccination, the fold changes in gene expression of (A) CD274 (PDL-1) in sorted CD11c cells and (C) CTLA4 in sorted CD4 cells isolated from specified organs was determined. Percentage of CD11c + cells expressing PDL-1 (B) or CD4 + cells expressing CTLA4 (D) were quantified in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (** p < 0.01, *** p < 0.001, **** p < 0 0001).

Journal: Frontiers in Immunology

Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice

doi: 10.3389/fimmu.2020.00712

Figure Lengend Snippet: Endogenous TGFβ alters PDL-1 and CTLA4 expression in secondary lymphoid tissues. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100) or Salmonella (pSBbi-RP/SB100). Four weeks post-vaccination, the fold changes in gene expression of (A) CD274 (PDL-1) in sorted CD11c cells and (C) CTLA4 in sorted CD4 cells isolated from specified organs was determined. Percentage of CD11c + cells expressing PDL-1 (B) or CD4 + cells expressing CTLA4 (D) were quantified in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (** p < 0.01, *** p < 0.001, **** p < 0 0001).

Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using PE-conjugated CD11c antibody (Miltenyi, 130-110-701) followed by anti-PE microbeads (Miltenyi Biotec, 130-091-041).

Techniques: Expressing, Gene Expression, Isolation

A Salmonella -based vaccine increase DCs in lymphoid organs. NOD mice were injected with 2.5 μg/mouse of anti-CD3 mAb for 5 days followed by 2 doses of oral vaccine containing PPI + TGFβ + IL10 ( n = 5). Four weeks post-vaccination, DCs were analyzed in different organs by FACS analysis. (A) Quantification of the percentage of CD11c + in total live cells of the spleen, PLN, MLN, and PP. (B) MLN, (C) PP, (D) PLN, and (E) spleen expression of CD80, CD86, and MHCII was measured in DCs from each organ. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between vaccine and vehicle treated groups (* p < 0.05, ** p < 0.01, **** p < 0.0001).

Journal: Frontiers in Immunology

Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice

doi: 10.3389/fimmu.2020.00712

Figure Lengend Snippet: A Salmonella -based vaccine increase DCs in lymphoid organs. NOD mice were injected with 2.5 μg/mouse of anti-CD3 mAb for 5 days followed by 2 doses of oral vaccine containing PPI + TGFβ + IL10 ( n = 5). Four weeks post-vaccination, DCs were analyzed in different organs by FACS analysis. (A) Quantification of the percentage of CD11c + in total live cells of the spleen, PLN, MLN, and PP. (B) MLN, (C) PP, (D) PLN, and (E) spleen expression of CD80, CD86, and MHCII was measured in DCs from each organ. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between vaccine and vehicle treated groups (* p < 0.05, ** p < 0.01, **** p < 0.0001).

Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using PE-conjugated CD11c antibody (Miltenyi, 130-110-701) followed by anti-PE microbeads (Miltenyi Biotec, 130-091-041).

Techniques: Injection, Expressing

List of cell surface markers used in this study

Journal: Journal for Immunotherapy of Cancer

Article Title: An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides

doi: 10.1186/s40425-018-0339-5

Figure Lengend Snippet: List of cell surface markers used in this study

Article Snippet: CD11c-VioBlue , Miltenyi , 130-102-797 , Myeloid Cell, DC.

Techniques: Marker, Activation Assay

List containing all antibodies utilized for surface staining of mass cytometry samples.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: List containing all antibodies utilized for surface staining of mass cytometry samples.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Staining, Mass Cytometry, Marker

General gating strategy for identified immune cell subsets and the used marker combination for definition.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: General gating strategy for identified immune cell subsets and the used marker combination for definition.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Marker

Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Two Tailed Test